MICROBIAL FACTORY 1

Innovative Lactic Acid

Cyanobacterium Synechocystis to produce lactic acid (LA) as a polylactate (PL) plastics precursor.

What we will do in a nutshell:

TRL 5 aerobic photobioreactor reliant on engineered Synechocystis strains to produce enantiopure L-lactic acid, which is the precursor of polylactate bioplastics, providing the best potential economic margin according to PHO, from CO2 and driven by solar radiation during daylight hours and/or LED radiation (e.g. during night-time hours). Operating the photobioreactor exploiting both solar and artificial light is enabled by the envisaged highly integrated processes’ scheme, which will power LEDs from low-price electricity derived from a biogas engine based cogeneration unit.

 

The main factors we want to capture in the concept design of the MF1 (arbitrarily ordered) are:

  • increase lactic acid tolerance;
  • elimination of competing substrate-consuming pathways;
  • increase of substrate availability for the lactate dehydrogenase (LDH) enzyme;
  • optimize expression of LDH using systems that respond to cues that are industrially relevant;
  • modulate activity of LDH using recently identified allosteric effectors;
  • increase photosynthetic efficiency of production strains by the broadening of their absorption spectrum;
  • optimize the minerals requirement during production
  • optimize/ select the optimal conditions for the purification of lactic acid.

The ultimate goal is to design strains, encompassing metabolic engineering strategies that are predicted to lead to stable and robust production of the target compound Lactic acid at the highest titres and productivities possible.

labsPHOTANOL

Advanced Chemicals

Skilled team

labs2
lab.pho

Innovative tech

eu-logo

This project has received funding from the European Union's Horizon 2020 Research and Innovation Programme under Grant Agreement No. 760994. 

The content of this publication is the sole responsibility of the authors. The European Commission or its services cannot be held responsible for any use that may be made of the information it contains.

© 2018 Engicoin
Privacy and Data Protection

IIT's website uses the following types of cookies: browsing/session, analytics, functional and third party cookies. Users can choose whether or not to accept the use of cookies and access the website. By clicking on "Further Information", the full information notice on the types of cookies will be displayed and you will be able to choose whether or not to accept them whilst browsing on the website.

Further Information I Understand